In this paper, the wind tunnel test was carried to investigate the behavior of buffer layer in turbulent boundary layer with variation of surface temperature and roughness. The results were as follows; 1. The velocity in turbulent boundary layer was increased when the roughness height within viscous sublayer thickness was increased. 2. When the surface temperature was increased, the density of air was decreased and the velocity in turbulent boundary layer was increased. Thus, the thickness of turbulent boundary layer was decreased. 3. When the roughness height and surface temperature was increased simultaneously, the thickness of turbulent boundary layer was decreased. 4. The decrement of the thickness of turbulent boundary layer was more effected by the increment of the roughness height rather than the increment of surface temperature. 5. In this study, it was found that the condition of the highest velocity n turbulent boundary layer was the temperature 333K and roughness #100.