This study was intended to investigate the effect on the human body such as subcutaneous fat thickness the circumference of extremities etc. of the clothing training of putting on thin clothes periodically from the cold period. The subjects were divided into the clothing training group and the non-training group, The training group was asked to wear cool clothes in daily life and to wear the training clothes of T-shirts with half-length sleeves and pants and perform the clothing training for two hours daily three times a week in a cold environment over the period from November to February. The non-training group was asked to lead a life wearing comfortable clothes. Then a comparative experiment was conducted at 15$pm$1$^{circ}C$, 50$pm$5% R, H and 0.25m/sec before and after the clothing training. After the clothing training regardless of gender subcutaneous fat thickness was more increased and total clothing weight per the surface area of the body was decreased in the training group than the non-training group. The training group showed lower skin temperature in the limbs and lower average skin temperature than the non-training group irrespective of gender which proved the effect of the clothing training. The training group was shown to have attendancy toward a greater sense of warmth and a less sense of discomfort which proved the effect of the clothing training.