This study was done to research the effects of temperature on biological evaluation of seawater quality using gametes, embryos and early development system of seaurchin species, Hemicentrotus pulcherrimus, Anthocidaris crassispina and Scaphechinus brevis. As the result of performing effects of temperature on early embryo development, the conditions of appropriate temperature on formation of normal pluteus were appropriate at 5-16$^{circ}C$ for H. pulcherrimus, 8-2$0^{circ}C$ for A. crassispina, 12-2$0^{circ}C$ for S. brevis. The conditions of optimum temperature on biological evaluation were 16$^{circ}C$ for H. pulcherrimus and 2$0^{circ}C$ for A. crassispina and S. brevis.