This study was carried out to examine the effect of clothing materials and multi-layered textiles on thermal resistance value. Cotton, polyester, wool, silk, rayon and acetate were selected for the specimens. Thermal resistance value was tested with 2 kinds of methods(thermo labo II and BK type tester). The results were as follows; 1. The effects of clothing materials for thermal resistance value were decreased by adding layers. 2. When the fabrics are measured with multiple layers, the fabric of the lowest thermal resistance value at single layer was showed the highest increasing tendency for all test methods.