In the replacement of bearing system of bridges, the jacking work to secure work spaces may cause damage of the superstructure, hence the behavior of superstructure by the jacking force must be considered. Especially, in prestressed concrete I-type girder bridges, considering the stress concentration at the girder and the load redistribution of superstructure, the allowable jacking force and jacking sequence have to be determined. In this study, an analytical method is proposed to calculate the jacking force and overall jacking sequence for the replacement of bearing system without any damage to the superstructure. The stress concentration at the girder and load redistribution of the deck due to jacking force are considered to compute the allowable jacking force for each girder and overall jacking sequence for girders in the deck. Using the solution algorithm developed in this study, the optimum jacking sequence and required jacking force for the prestressed concrete I-type gilder bridge having the standard sections are calculated.