One day to one week-old 3 female Korean native calves were referred to the Veterinary Teaching Hospital, Chonbuk National University, with atresia ani. The authors performed radiographic and ultrasonographic diagnosis with physical examination, general hematology and blood chemical examination as investigation. The atresia ani were type I, II, III in each 3 calves. In abdominal radiography and ultrasonography, there were ventral displacement of descending colon(gas and feces filled) and typical enlargement of the blind end of colon in all cases. Operation site was determined by radiographic and ultrasonographic findings in these cases. Surgical treatment, two cases were translocation of the colon to the body wall exiting as a colostomy and one case was corrected by making a circumcision through the skin covering the site of the anus.