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신생 횐쥐의 기관섬모에서 $alpha$-tubulin의 발현과 섬모형성 양상에 대한 면역전자현미경적 연구
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  • 신생 횐쥐의 기관섬모에서 $alpha$-tubulin의 발현과 섬모형성 양상에 대한 면역전자현미경적 연구
  • An Immuno-Electron Microscopic Study of Expression of $alpha$-tubulin on Ciliogenesis in Newborn Rat Trachea
저자명
김보형,진정언,임대준,김동욱,이근수,강성호
간행물명
大韓氣管食道科學會誌
권/호정보
1999년|5권 1호|pp.42-48 (7 pages)
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대한기관식도과학회
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

Background: This study was undertaken to detect the patterns of ciliogenesis in newborn rats trachea. Materials and Methods: The experimental animals(Sprague-Dawley strain) were divided five groups, one day, two day, three day, five day and seven day newborns as experimental groups. To obtain differential distribution of $alpha$-tubulins in the ciliated cells and patterns of ciliogenesis, we used immunohistochemical stain method with mouse anti $alpha$-tubulin monoclonal antibody as the primary antibody and gold particles conjugated goat anti mouse IgG as the secondary antibody. And we observed the specimens by electron microscope (Hitach-600 Model). Results : 1) The distribution of the $alpha$ -tubulin reactions in apical zone was slightly decreased from three day after birth. 2) From 5th day after birth, the decreasing number of gold particles in intermediate zone was remarkable. 3) On the comparison with the other zones, the number of gold particles in the Golgi zone for seven days showed no statistical difference. Conclusion : The ciliogenesis of the tracheal ciliated cells in early newborn rat were made via centriolar and acentriolar pathways, in late groups, from five day after birth, the major ciliogenesis pattern might be centriolar pathway.