This paper investigates ventilation characteristics of an environmental chamber simulating an under-floor air conditioning system for isothermal and cooling supply air conditions. The tracer gas sulfur-hexafluoride (SF$F_6$) was injected into a supply duct using step-up and step-down methods. Local mean and room mean ages were calculated from the concentrations measured at internal points and at the exhaust duct. The air change efficiency of the chamber has been found to be greater in cooling conditions than in isothermal conditions. Also the room air change efficiency is not significantly affected but slightly improved by the presence of a supply diffuser.