Objectives : The purpose of this study was to systematically analyze and compare the acoustic characteristics of vocal major students with vocal nodule when singing. Materials and Methods : Ten sopranos with vocal nodule, who have never been treated due to voice problems, were the subject group. Twenty healthy sopranos major students were the control group for this study. The vocal nodule was confirmed by stroboscopy and the acoustic and electroglottographic analysis was conducted on be groups. Additionally, an inquiry on usual voice problems during speaking and singing were performed on each of the groups. Results : The vowel /a/ was spoken and sung by sopranos of each group. There were no significant differences in Fo, jitter, shimmer, NHR at speaking and singing in either group. We did not register any significant differences of closed quotient and speed quotient in electroglottography for both groups at singing, but speed quotient was increased in subject group at speaking(p＜0.05). And the sopranos with nodule demonstrated voice fatigue(70%), strained and tired voice after long period of performance and conversation(60%) on inquiry. Conclusions : Although they had a vocal nodule, this group, who had a long period of voice training, did not present any differences in acoustic and electroglottographic problems during short performance, but, the vocalists noticed delicate voice problems after a long performance. We conclude that the voice training teacher and laryngologist must initiate voice management and speech therapy for vocal nodule, even if there is no specific abnormality in acoustic analysis.