Background : This study was conducted to reduce the neonatal infection rate in nursery. Methods : 50 items, structural problems in NICU were selected by open questionnaire and categorized into 11 similar items. 38 items were picked out among them and then categorized into 6 similar items. We carried out quality improvement focusing around 6 items. Results : The high achievement of goal in CQI Activities was shown in hand washing for reducing infection of NICU after July. The local infection in IV site was not found and hand washing and gowning of protectors were achieved by 100%. Conclusion : Neonatal infection rate was reduced through the quality improvement activities, but structural problem of Hospital still remained.