Many industrial products can be assembled in various sequences of assembly operations. To save time and cost in assembly process and to increase the quality of products, it is very important to choose an optimal assembly sequence. In this paper, we propose a methodology that generates an optimal assembly sequence by using the knowledge of experts. First, a product is divided into several sub-assemblies. Next, the disassembly sequences of sub-assembly are generated using disassembly rules and special information can be extracted through the disassembly process. By combining every assembly sequence of sub-assemblies, we can generate all the possible assembly sequences of a product. Finally, the expert system evaluates all the possible assembly sequences and finds an optimal assembly sequence. It can be achieved under consideration of the parameters such as assembly operation, tool change, safety of part. basepart location, setup change, distance, and orientation. The developed system is applied to UBR(Unit Bath Room) example.