This study was conducted to identify the effects of high-heel shoes on EMG activities of rectus femoris and biceps femoris in 28 healthy women. Subjects were composed of experimental group (wearing high-heel shoes) and control group (wearing low-heel shoes). Two groups participated in three conditions standing (bare foot wearing athletic shoes and 7.5 cm height shoes). In high-heel shoes condition, EMG activities of rectus femoris of control group were significantly lower than that of biceps femoris of experimental group, but EMG activities of both muscles of experimental group did not should significant difference. In bare foot standing condition, EMG activities of rectus femoris of experimental group were significantly lower than that of biceps femoris but EMG activities of both muscles of control group had no significant difference. These results showed that hamstring lengthening effects was produced when wearing high-heel shoes because the external knee extension moment was increased. In the short term, high-heel shoes effect on the increase of the biceps femoris activities by spindle reflex, but in the long term, the normal amplitude of the same muscle activities by Golgi tendon organ reflex.