Objectives: To determine the effect of interpolation functions when processing the digital periapical images. Material and Methods: The digital images were obtained by Digora and CDR system on the dry skull and human subject. 3 oral radiologists evaluated the 3 portions of each processed image using 7 interpolation methods, and ROC curves were obtained by trapezoidal methods. Results: The heighest Az value(0.96) was obtained with cubic spline method and the lowest Az value(0.03) was obtained with facet model method in Digora system. The heighest Az value (0.79) was obtained with gray segment expansion method and the lowest Az value(0.07) was obtained with facet model method in CDR system. There was significant difference of Az value in original image between Digora and CDR system at a=0.05 level. There were significant differences of Az values between Digora and CDR images with cubic spline method, facet model method, linear interpolation method and non-linear interpolation method at α=0.1 level.