The influences of fluorophor, scatterer, and absorber in turbid material by light scattering were interpreted for the scattered fluorescence intensity and wavelength. The molecular properties have been studied by laser induced fluorescence spectroscopy in phantom. It has been found that the effects of optical properties in scattering media could be investigated by the optical parameters(${mu}_a$, ${mu}_a$, ${mu}_t$). Experimental and Monte Carlo Simulation method for modelling light transport in tissue was applied. The experimental results using a phantom were discussed and compared with those obtained through Monte Carlo Simulation. It may also aid in designing the best model for oil chemistry, medicine and application of medical engineering.