This study conducted 4 different kinds of underwear materials, which were A (Cotton 100%), B (Wool 100%), C (Cotton/Wool, 50/50%) and D (Acrylic/Cotton, 50/50%) and were done in a climate chamber under cold ambient $10{pm}1^{circ}C$, $40{pm}5%RH$ by 6 male subjects who were in good health. Physiological parameters such as rectal and local skin temperature(forehead, forearm, hand, trunk, thigh, leg, foot, back and chest), heart rate, body weight loss, clothing microclimate, blood lactic acid concentration, and wearing sensation were measured. Started with a 15-min rest period, 15-min of exercise 1 (the condition of 4.5 mile/hr walking speed equivalent to with 8.5 Kcal energy consumption on the treadmill) period, 15-min rest period, exercise 2 (after 3minutes warming-up at 3.0. 3.7, 4.5. 5.2. 6.0, 6.7 mile/hr) until exhaustion period, and final 15-min of recovery period were performed. The results were as follows: The lowest mean skin temperature was acrylic/cotton in order of wool > cotton/wool > cotton > acrylic/cotton (F=13. 79. p<0.00l). Most of all skin temperature by parts of body had turned out in sequence of temperature wool > cotton/wool > acrylic/cotton > cotton. Fore arm part showed highest temperature about $32.43^{circ}C$ on wool and had a tendency approximately $1.8^{circ}C$ higher than cotton which had the lowest temperature, and had the biggest difference among garments in terms of skin temperature. The back temperature within clothing showed about $2^{circ}C$ higher than the chest temperature within clothing. but the back humidity within clothing showed about 4~12% higher than the chest humidity within clothing. Body weight loss by each garment was this sequence; cotton > acrylic/cotton > wool > cotton/wool.