Seasonal variation in species composition of fish with depth was determined by analysis of bimonthly samples collected by a beach seine at the shallow water (St. 1 < 1.5m) and by beam trawl at the two stations (St. 2, 5~7m and St. 3 > 15 m) from October 1997 to August 1998 off Ippa-do in Asan Bay. Of forty species identified, 13 species at St. 1,28 species at the St. 2 and 30 species at the St. 3 were collected. The fish density was also increased with depth. Favonigobius gymnauchen occupied 55.7% at St. 1 and 38.9% of the number of individuals at St. 2. Almost of fish collected at these two stations were juveniles, and they were principally collected in August and in November. At St. 3, relatively large fishes were collected. Among them Pholis fangi and Chaeturichthys stigmatias predominated in the number of individuals. Abundance was low, but a large number of species were collected in winter. Principal component analysis revealed that the species composition at the shallower stations was different from that at the deeper station. The number of species and abundance of fish in Asan Bay was lower than in the other western coastal waters of Korea. Species composition in the study area of sandy bottom was different from that in the inner Asan Bay of mud bottom.