Acycloretinal을 Tollens 시약으로 처리하여 acycloretinoic acid를 얻었다. 생성된 표준품 acycloretinoic acid는 Silica-HPLC 및 ODS-HPLC를 통하여 정제를 행하고 GC-MS에 의해 동정하였다. Lycopene을 toluene과 Tween 40 수용액에서 $37^{circ}C$ 조건으로 자동산화시킨 결과, acidic 화합물이 생성되었다. Lycopene의 자동산화로부터 생성된 acidic 화합물은 그 HPLC상의 거동, 분광학적 특성 및 mass spectra의 특성이 acycloretinoic acid 표준품과 잘 일치하였다. 이러한 결과로 in vitro상의 산화적 조건하에서 lycopene의 자동산화에 의해 acycloretinoic acid가 생성됨을 알 수 있었다.
Acycloretinoic acid was prepared from acycloretinal by oxidation with Tollens reagent. Acycloretinoic acid was separated with Silica-HPLC and analyzed by ODS-HPLC with a photodiode array detector and by GC-MS. Lycopene was solubilized in toluene and aqueous Tween 40, and then oxidized by incubating at $37^{circ}C$ under atmospheric oxygen. Acidic compound was produced by autoxidation of lycopene. Retention time, UV-Vis spectra and mass spectra of the acidic compound were identical to the standard acycloretinoic acid. Thus, acycloretinoic acid was confirmed to occur in vitro under oxidation condition of lycopene.
