The purpose of this study was to determine the overflow effect and cross training effect of isometric quadriceps training that performed in specific angle of unilateral let. Ten healthy students with an average age of 24 years$(24.1pm1.3)$, were participated in this study. Then 5 subjects in each group were chosen at random to train using only right quadriceps muscle two time per day(group 2), five times a week and the other 5 subjects(group 1) were chosen to train one times per day, five times a week for 2 weeks at only 50 degrees (contract 6 seconds, rest 10 seconds, 3 sets). Before and after the training, isometric quadriceps muscle testing of the both leg was Performed at three different angles, 60, 50 and 40 degrees respectively by BHN-COM (isokinetic dynamometer) in sitting position. The data was analyzed with paired t-test to determine significant difference between before and after training. In this study, we have found that the isometric quadriceps muscle training on specific angle of right side produced overflow effect In healthy subjects. However, increasing the peak torque of specific angle(training angle) of trained limb did not have an effect on increasing the peak torque of contralateral limb. These results demonstrate that the cross training effect did nut found in this study but a alight increase of peak torque of the untrained limb would recognized the possibility of cross training effect.