The primary cause of air pollution by vehicles is imperfect combustion of fuel. One of the most usual causes of this imperfect combustion is the misfire in IC(Intenal Combustion) engine. Recently it is obligated for an ECU to monitor the emission level and warn the driver in case of exceeding specified emission standards. Therefore, in order to comply with this OBD-II regulations, car makers are investing a considerable amount into technology which would enable the detection of misfire and the particular cylinder in which misfire is taking place. So far, it has been able to detect misfire using engine speed, which can be obtained crank angle. However, such a method posed a problem in analyzing at high speed and in recognizing the misfire from the load impact at bumpy road. In this paper, misfire detection is made possible by simple arithmetic using WDFT, especially at high engine speed. In addition, the moving window method of a Walsh function is applied to determine the cylinders under misfire in case of multiple misfires. An actual experiment was conducted to prove that WDFT is applicable to effective in computation speed and to same result in misfire detection and cylinder determination at idle, part load and bumpy road conditions.