Methanol extract of Sarcodon aspratus Berk. (S. Ito) was analyzed by thi layer chromatography, gas chromatography and mass spectrophotometer. Nine fractions from primary methanol extract were observed by TLC. Six fractions were observed by the second TLC analysis of the second and the third fractions. 27,28-digydrolanosterol({TEX}$C_{30}H_{52}${/TEX}O), ergost-7-en-3-ol({TEX}$C_{28}H_{48}${/TEX}O) were identified from two fractions of the second TLC analysis by mass spectrophotometer. The molecular weights of 27,28-dihydrolanosterol and ergost-7-en-3-ol were 413 and 400 respectively.