This paper deals with the development of biomechanical model on a seat with backrest support in the vertical direction. Four kinds of biomechanical models are discussed to depict human motion. One DOF model mainly describes z-axis motion of hip, two and three DOF models describe z-axis of hip and head, and while nine DOF model suggested in this study represents more motion than the otehr model. Three kinds of experiments were executed to validate these models. The first one was to measure the acceleration of the floor and hip surface in z-axis, the back surface in x-axis, and the head in z-axis under exciter. From this measurement, the transmissiblities of each subject were obtained. The second one was the measurement of the joint position by the device having pointer and the measurement of contact position between the human body and the seat by body pressure distribution. The third one was the measurement of the seat and back cushion by dummy. The biomechanical model parameters were obtained by matching the simulated to the experimental transmissiblities at the hip, back, and head.