The primary objective of this study is to estimate the suitable flow in need for conservation and restoration of the fish habitat in running water ecosystem, which has very important status in the instream flow for stream environment. Year, monthly low flows are estimated to properly maintain the fish habitat. Water depth and velocity are simulated, and also water temperature and Dissolved Oxygen(DO) are predicted at gradually varied flow using estimated low flows. These simulated conditions for each low flow are graphically compared with the requirements to maintain fish habitat at each life stage. These processes were applied to 3 riffle transects located at Dalcheon(Dal stream) in the South Han river. Pirami (Zacco platypus) was selected as a representative fish species in Dalcheon. It was shown that the suitable flow for maintaining the representative fish habitat at each life stage depends on hydraulic conditions rather than water quality conditions, and the flow ranges from the 10-year minimum low flow to consecutive 7-day 2.33-year low flow.w flow.