A bactirium prohibiting the growth of fungus Botrytis cineria KT 433 was isolate and identified from soil. The isolated strain was gram positive, aerobic bacteria with cream color, round and mucoid. It showed ord form of 0.45$ imes$1.1 ${mu}{ extrm}{m}$ at the cultivated for 24 hrs and the ellipsoided endospore wer observed after culting for 72 hrs. The optimum growth temperature and pH wer 35$^{circ}C$ and pH 5.0~8.0, respectively. It could assimilate daxtrin, maltose, glucose, mannose, ribose and collobiose as a sole carbon source. The isolated was confirmed to be a Bacillus sp. strain from the findings. The antibiotic from the isolated strain was stable up to 121$^{circ}C$. The strain, especially, showed specific activity for mold and yeast. However, there was not significant antibacterial activity.