This study was to investigate antiallergic contact hypersensitivity of the polyphenol fractons isolated from persimmon leaves (Diospyros kaki folum). The result of the allergic contact dermatitis is a common skin disease resulting from specific immunologic sensitization due to topically applied allergens. Here the contact hypersensitivity was assayed and obdominal skin morphological changes including mast cells were examined. At contact hypersensitivity assay, less right ear swelling occured in the polyphenol fraction I, II and III groups compared to the control group. The number of mast cells was significantly decreased in the sample groups than the control group.