Dyeing fastness and antibiosis effects of fabrics dyed with wormwood, which has been used in folk medicines and dietary life in Korea for a long time, were studied through dyeing small overcoats, which are used as diapers, with wormwood extract. As for the dying fastness test results, the fastness of decoloration was not that good but the pollution level showed a relatively positive result. As for the antibiosis effect results, the antibiosis of the dyed sample was better than the original sample on 3 kinds of bacteria, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis and on 2 kinds of the fungi(Eumycetes). Aspergillus niger and Candida albicans. Especially, the sample that was mordant with CaCl2 and FeSO4·7H2O showed the best antibiosis effect. On the other hand, the sample that showed the best antibiosis effect was the refined fabric, which is a fabric that is not dyed.