This study was carried out to investigate the effect of light intensity on the growth and biomass production of Pinus thunbergii seedlings. The experiment was performed under five different light intensities such as 100, 50, 30, 10, and 25 of the natural full sun light intensity for 2 year in the field condition. The seedling showed the highest height and root collar diameter growth under natural full sun light. The height growth of the seedling under 50% of light intensity showed 96.4% of the control seedling in the first year, and 88.9% in the second year, and the root collar diameter growth showed 94.1% in the first year 77.6% in the second year. Height and root collar diameter growth was markedly reduced below 30% of relative light intensity, and the reduction rate of root collar diameter growth was higher than that of height growth. All the tested seedlings died under 2% of relative light intensity in the second year. Biomass production was also reduced by decreasing of relative light intensity, the reduction rate of root biomass production in both first and second years was higher than that of leaf or shoot biomass production. The lowest reduction was observed at the shoot in the first year, and at the leaves in the second year The highest T/R ratio was recorded by 3.55 in the seedling under 50% of relative light intesity in the first year, and by 4.88 under 10% of relative light intensity in the second year.