The purpose of this study is to evaluate naturalness of botanical garden pre- and post-assessed according to the Hemeroby Index in the case study of Daegok Botanical Garden. The results of the study are as follows. 1) The results of the site analysis according to the Hemeroby Index before development have been appeared that highest of poly/meta-hemeroby is 45.8% and the next of meso-hemeroby is 28.3%. 2) After development, the highest of meso-hemeroby is 53.6% and the next of $alpha$ -euhemeroby is 28.3%. Generally, it is considered that the naturalness according to Hemeroby Indx was lasted higher after development than that before development. 3) Both a-hemeroby and oligo-hemeroby are not appeared before and after development. $alpha$ -euhemeroby and meta-hemeroby are not appeared before development, but htey increased as much as 16.8% and 6.8% after development. Most of all, the increase of meta-hemeroby after development results from the increase of the artificial facilities (e.g. pavements, buildings and so on). 4) Specially, through the modification of play ground, park lot, pavement classified to meta-hemeroby, it is considered that the Hemeroby Index would be improved. 5) Compared with existing naturalness grade of green space, it has merits to apply Hemeroby Index, especially as a menas of distinguised naturalness assessment in various land use pattern. But there is no clear evidence that limits the minimum size that could be applied with Hemeroby Index. So, we must discuss whether it was useful to apply this standard to such a small specific space as the site of this study.