Experimental and computational studies were carried out to confirm the turbulent enhancement of the cooling system of nuclear reactor by large scale vortex generation in nuclear fuel bundle. The large scale vortex motions were generated by rearranging the inclination angles of mixing vanes to the coordinate directions. Axial development of mean and turbulent velocities in the subchannels were measured by the 2-color LDV system. Eddy diffusivity heat flux model and $k-varepsilon$ model were employed to analyze the turbulent heat and fluid flows in the subchannel. The turbulence generated by split mixing vanes has small length scales so that they maintain only about $10 D_H$ after the spacer grid. On the other hand, the turbulences generated by the large scale vortex continue more and remain up to $25 D_H$after the spacer gird.