This study estimates the pollution load in a basin by regional groups analyzing the relationship between the discharge and pollution load. The study area is placed in the Miho stream basin known as the main tributary of the Kum river. Four major Telemetary streamflow stations are chosen. In this research, discharge and water quality in a dry season and a flood season from the observed discharge in the stream are analyzed. The Rating-Curve and the Pollutograph are drawn analyzing discharge and water quality at the major stations. The characteristics of runoff for each stream are analyzed and the change of water quality are analyzed for rainfall period. The relationship between discharge and water quality has been investigated. The relationship between the discharge and pollution load is analyzed and a representative equation is derived. These relationships permit an estimates of the pollution load at the Miho stream basin. basin.