A frequency assignment problem on time division duplex system is considered. Republic of Korea Army (ROKA) has been establishing an infrastructure of tactical communication (SPIDER) system for next generation and it will be a core network structure of system. VHF system is the backbone network of SPIDER, that performs transmission of data such as voice, text and images. So, it is a significant problem finding the frequency assignment with no interference under very restricted resource environment. With a given arbitrary configuration of communications network, we find a feasible solution that guarantees communication without interference between sites and relay stations. We formulate a frequency assignment problem as an Integer Programming model, which has NP-hard complexity. To find the assignment results within a reasonable time, we take a genetic algorithm approach which represents the solution structure with available frequency order, and develop a genetic operation strategies. Computational result shows that the network configuration of SPIDER can be solved efficiently within a very short time.