For maximum seeding efficiency of a nozzle type seeder, the performance of the nozzle should be considered sufficiently. This study was carried out to investigate the optimum operating conditions of a seeder attached the vacuum nozzle which was modified syringe needle acting on the plug seedling tray and the seed plate. Such operating factors as the hole diameter of the nozzle (d), the distance from the nozzle tip to the bottom plate of seed hopper(D) the absorbing air pressure of the nozzle tip(P) the bounding height of seed from the vibrated bottom plate of seed hopper and the seeding speed were selected based on the weight of a grain of seed(W). The treated materials were pepper seed as the flat type, cucumber seed as the oval type and radish seed as the spherical type. The optimum operating conditions of the experimental seeder were revealed as follows: 1. The height of the seed bounding from the bottom plate of seed hopper and the distance from nozzle tip to bounded seed were 5 mm and 0.5 mm at all seeds. The hole diameter of the nozzle and the absorbing pressure for pepper seed, cucumber seed and radish seed was 0.45 mm, 0.65 mm. 0.65mm and 39.2 kPa, 88.3 kPa, 58.8 kPa, respectively. 2. The absorbing pressure P was represented as P=η.4W/$pi$d$^2$ where η was 100. The seeding speed using a 128 cell tray was 2.4 cm/s which was same transfer as 2.5 trays per minute. 3. The maximum seeding rate in case of the pepper seed was 97% the cucumber seed was 95% and the radish seed was 100% under the optimum operating conditions of the seeder.