This study is concerned with evaluating the effects of acoustic excitation on the development of two stream mixing layer generated by split plate. The ratios of two velocities U1 and U2 either side of the splitter plate were such that $U_1/U_2$=1.0 (uniform flow) or $U_1/U_2$<1.0(shear flow). The mixing layers were disturbed acoustically through the edge of split plate. Quantitative data were obtained with hot-wire anemometry. Flow visualization with smoke-wire was also employed for qualitative study. the results show that the large scale structures of mixing layers are strongly affected by excitation frequency and amplitude in both uniform and shear flows. The maximum streamwise and vertical turbulent intensities of the excited flow fields are apt to be decreased as compared with those of without excitation. The flow characteristics of uniform flow are more influenced by acoustic excitation than those of shear flow.