An experimental study on the stratified fluids was carried out to investigate the flow characteristics, heat transfer through the interface of fluids, and the applications of thermal behaviors in the square cavity. The same volume of water and silicon oil was filled in it. The experiments were performed with the variations of initial temperatures and cooling surface temperatures. And the effect of vertical or horizontal cooling surface positions in the square cavity was investigated. When the cooling surface temperatures were $-4^{circ}C$ , the supercooling phenomena were observed for both cases of cooling surface positions in the full region regardless of the initial temperature of fluid. In the square cavity with horizontal cooling surfaces, the lower the initial temperatures were, the longer the supercooling durations were, and with vertical cooling surface the lower the initial temperatures were, the shorter the supercooling durations were.