A new algorithm for estimating bladder volume for portable bladder volume measurement system is proposed. Given the actual image of a bladder, edges between bladder wall and urine are extracted first. Axes are calculated from these data and actual cross section shape is obtained by filtering. Since ordinary shape of a bladder is irregular, two cross-sections(transverse and longitudinal) are considered. With the area of a longitudinal cross-section projected along the axes of transverse cross-section, or vice versa, two estimated volume are obtained. Averaging these two value yields the volume of the bladder. Applied to actual experiments, the proposed algorithm showed explicitly good results in comparison with the conventional techniques.