It is a serious subject for energy conservation to prevent the energy loss caused by mixing of heated and cooled air jets in a building which two types of air-conditioning systems are adopted in perimeter and interior zone. The purpose of this paper is to clarify the quantitative and qualitative mechanisms of the mixing loss and to propose preventive methods for it. In this paper, by using the dynamic heat load calculation method, heat extraction loads of a typical office building in Pusan are calculated. According to the results, numerical simulation based on the computational fluid dynamics were peformed in order to measure the mixing loss in physical size HVAC system. Then, the distributions of air temperature and velocity are analyzed in order to grasp the relations by setting temperature differences influence on the mixing loss.