Apolipophorin-III(ApoLp-III)를 박각시나방(Agrius convolvuli)의 종령유충 혈림프로부터 gelpermeation chromatography (Sephadex G-100)와 ion exchange chromatography (DE-52)를 이용하여 분리, 정제하였다. 정제된 apoLp-III의 분자량은 12% SDS 전기 영동상에서 20 kDa로 측정되었으며, 또한, citcular dichroism spectrum측정에 의하여 상당한 양의 $alpha$-helica1 2차 구조를 지니고 있는 것으로 밝혀졌다.
Apolipophorin-III (apoLp-III) was isolated and purified from the last instar larval hemolymph of Agrius convolvuli by gel permeation chromatography (Sephadex G-100) and ion exchange chromatography (DE-52). The molecular mass of apoLp-III determined to be 20 kDa using 12% SDS polyacrylamide gel electrophoresis. The circular dichroism spectrum from the purified apoLp-III indicated a considerable content of $alpha$-helical secondary structure.
