The identity of biotypes of Bemisia tabaci, recently found in Korea, were determined using a partial sequence of its mitochondrial 16S rRNA. The samples of B. tabaci collected from Gtycine max (KRG), Euphorbia pulchenima (KRP), and Rosa hybpida (KBR) were compared with seven previously identified populations from Japan, Israel, Australia, and the USA. The sequences from both KRP and KRR were identical to those of B biotypes distributed in Arizona, Australia, Israel, and Japan. The sequence obtained from KRG was identical to that obtained from a population on Shikoku Island, Japan. These two populations are non-B biotype and in an earlier study have been shown to belong to the same clade as populations from Hainan, Turkey and northern India. A third distinct genotype was also found in a population from Ishigaki Island, Japan. The genetic distance in pairwise comparison and a phylogenetic tree inferred from 16S rRNA sequences are shown.