Sixty local soybean cultivars were evaluated on the antioxidative activity by superoxide dismutase (SOD), 1, 1-diphenyl-2-picrylhydrazyl (DPPH), thiobarbituric acid(TBA), and chemiluminescence using the FI-CL system. Soybean were collected throughout the country, and were grown over two years (1997 and 1998) for measuring antioxidative activity in soybean seeds. There were differences in antioxidative activity depen-ding on the method of measurement and variation of the crop year. Soybeans from Kwangyang-shi-1 (76.78%) in 1997 and Kangjin-gun-3(79.14%) in 1998 showed the highest SOD activity, whereas those from Hwasoon-gun (80.43%) in 1997 and Kangjin-gun-2 (49.82 %) in 1998 exhibited the highest DPPH activity. Soybeans from Chongup-gun-2 (75.77%) in 1997 and from Yochon-shi-5 (69.17%) in 1998 exhibited the highest TBA activity, and those from Jinahn-gun (48.99%) in 1997 and Kohung-gun (49.73%) in 1998 exhibited the highest activity using the chemiluminescence method. These results suggest that it may be possible to develop soybean varieties with higher antioxidative activity.