The purpose of this study is to elucidate the source of U anomaly formed in stream water of the drainage system around the Shinbo talc mine area based on the O, H, S and Sr isotopic characteristics of water masses and wall rocks. The ${delta}$D and ${delta}^{18}O$ of surface and ground waters show highly restricted range and plotted on the same meteoric water line, indicating that they are all originated from the meteoric water. The ${delta}^{34}S$value of the ground water containing high U shows slightly negative (-0.2${ extperthousand}$) and quite distinct from those of the other surface and ground waters that are similar to those of wall rocks (>5.8${ extperthousand}$), indicating that they have a different S isotopic fractionation or less probably, source. The $^{87}Sr/^{86}{Sr}$ratios of water masses around the Shinbo talc mine area show a variable range from 0.724325 to 0.744928, but tend to increase with increasing U concentration of water mass. Although it is not possible to determine precisely the source rock of U anomaly formed in the hydrologic system around the Shinbo talc mine, the evidence obtained from the Sr isotopic compositions strongly suggests that coal schist and/or pegmatite vein could be the most likely candidate for the source rock.