The effects of two phosphorus sources (fused phosphate and rock phosphate), applied at different rates, on growth, arbuscular-mycorrhizae(AM) formation in roots and nutrient contents of trifoliate orange grown in an uncultivated volcanic ash soil were investigated in a greenhouse. The seedlings were either inoculated with AM fungi or left uninnoculated. Growth of seedlings were best in the treatments of 156-272 mg P/kg with fused phosphate. Although the applied P in the rack phosphate treatments were nearly same or much higher comparing to the fused phosphate treatments, seedling growth were significantly less. Soil available P in the treatment of 272 mg P/kg of fused phosphate was maintained in the range of 3-5 mg/kg during the experiment, and the AM formation was about 60% in average. In the treatments of lower rates of fused phosphate application or of rock phosphate application, soil available P were lower than 3 mg P/kg and AM formations were less than 30%. Significant increases were found in seedling growth and nutrient absorption due to AM fungi inoculation, and the effects were much more significant in the treatments of higher AM formation. In most of citrus groves in Cheju island, soil available P is much higher than 200 mg P/kg, and average AM formation in citrus roots is less than 30%. Results obtained in this study show that the formation of AM can be increased at much lower level of available P than the present levels found in citrus groves.