The purpose of this study were to assess the effect on health promotion behavior of health instruction in elementary school and to provide a basis of school health education. Subjects of this study were random sampled 80 students for control group in elementary 6th grade students. the treatment group systematically studied five sphere instruction: individual hygiene, disease prevention, first-aid & safety, oral health, health & nutrition in two hours a week, from April 20, till July 10. Each group was pre-tested and post-tested by health behavior promotion questionnaire. The conclusions as follows; 1. Compare analysis of control group and treatment group in the pre-test according to health condition of children were no significant difference physical health condition, health anxiety, disease-absent(P>.05). 2. Health promotion behaviors in the post-test according to concern quotient significantly correlated with individual hygiene(r=.249, P<.05), disease prevention(r=.477, P<.01), oral health(r=.228, P<.05), health & nutrition(r=.323, P<.01) in the treatment group, on the contrary the disease prevention, first-aid & safety, oral health, health & nutrition(P>.05). 3. The difference of health promotion behavior within & between group were as follow; 1) The difference of individual health hygiene with in the treatment group was significant in pre-post test, the comparison of the two groups was significant difference in post-test(treatment group: $3.965{pm}.568$, control group: $3.645{pm}.573$). 2) The difference of disease prevention with in the treatment group was significant in pre-post test, the comparison of the two groups was significant difference in post-test(t group : $3.475{pm}.619$, c group: $3.964{pm}.600$). 3) The difference of first-aid & safety with in the treatment group was significant in pre-post test, the comparison of the two groups was significant difference in post-test(t group : $3.700{pm}.466$, c group: $4.140{pm}.545$). 4) The difference of oral health with in the treatment group was significant in pre-post test, the comparison of the two groups was significant difference in post-test(t group: $3.163{pm}.665$, c group: $3.753{pm}.544$). 5) The difference of health & nutrition with in the treatment group was significant in pre-post test, the comparison of the two groups was significant difference in post-test(t group : $3.255{pm}.515$, c group: $3.698{pm}.558$).