참서대 암컷의 생식소중량지수는 6월에 가장 높았다. 난모세포의 발달양식은 난군동시발달형이며, 초기 성장기 난모세포에서 난병과 난황핵이 관찰되었다. 생식주기는 성장기 (2${~}$5월), 성숙기 (5${~}$6월), 완숙 및 산란기 ($6{~}8$월) 그리고 회복 및 휴지기 ($8{~}2$월)로 구분된다 전장 $TL 28.1{~}30.8 cm$의 개체당 절대포란수는 2,197 개였으며, 체중 g당 상대포란수는 10.0개였다.
Reproductive biology of the female red tongue sole, Cynoglossus joyneri was investigated by means of histological methods. Gonadosomatic index (GSI) was the highest in June. Developmental pattern of oocytes was group-synchronous. Egg stalk and yolk nucleus were observed in the early growing oocytes. Reproductive cycle could be classified into the growing ($February{~}May$), maturation ($May{~}June$), ripe and spent ($June{~}August$), and recovery and resting ($August{~}February$). The absolute fecundity per individual ($TL 28.1{~}30.8 cm$) was 2,197 and relative fecundity per g body weight was 18.0.
