The purpose of this study was to examine the knowledge and practice of the Clean Intermittent Self Catheterization (CIC) conducted by Spinal Cord Injury Patients (SPIC) or their significant others as an activity of daily living at their homes or working places. Forty-seven patients were participated in this study. Three questionnaires developed by researchers for this study were used to collect demographic data, knowledge and practice skill of CIC. Descriptive statistics and ANOVA were used to analyze the data. Nineteen (19) patients experienced Urinary Track Infection (UTI) during the last one years and two of them have had several times of UTI. Knowledge level of the participant was not high enough to perform correct CIC. Some evidences indicated that the knowledge did not apply to their practice. Systematic analysis and development of educational content based on this study results was recommended and the systematic instructional design before actual patient education was strongly recommended.