The purpose of this study is for evaluating the effect of MS Coat desensitizing agent in clinical situation. In this study total 60 teeth of 30 patients who is feeling hypersensitivity after periodontal surgery were treated with MS Coat desensitizing agent, and 20 teeth with saline solution for placebo effects and evaluated. All teeth were cleaned with rubber cup and pumice, after then the antibacterial agent was applied for 20 seconds and simply isolated using cotton roll Desensitizing agent was applied with a specific instrument in the manufacturers package by abrading motion for 10 seconds and re-done 8 times. Tactile stimulus with sharp explorer, air stimulus with syringe of dental unit, $7^{circ}C$ cold water stimulus using micropipette applicator was done to evaluate hypersensitivity before apply test agent, immediate after application, 1 week after, and 3 month after application. The degree of hypersensitivity was recorded using 100mm Visual Analogue Scale and compared between group and evaluation times. From this clinical study sensitivity to the stimulus was significantly induced in both groups and MS Coat made a significant reduction in VAS score than placebo group did(p<.01). The results of this study could demonstrate that MS Coat desensitizing agent can use effectively to heat dentinal hypersensitivity.