This study was carried out to investigate the effects of electrical stimulation on the change Tyrosine-Hydroxylase immunoreactive(TH-IR) cells in the Central gray and retrorubral field of the male SD rats. 9 healthy and normal rats were divided into three groups, 3 SD rat in each group. The one group has been stimulated by electroacupuncture(EA, 2Hz) for 30min and the other group by EA for 1hr 30min and control group has been stimulated. TH-IR cells were found in the Central gray(CG) and Retrorubral field(RF). The numbers of TH-IR cells of CG and RF were significantly increased after 30 min (CG $6.2{pm}0.83$, RF $1.4{pm}0.55$)as compared with control group(CG $24{pm}3.16$, RF $6.4{pm}0.55$) and were also significantly increased after 1hr 30min(CG $75.6{pm}4.51$, RF $18.8{pm}0.89$) than 30min. These results show that TH is related into CG and RF in response to electrical stimulation.