- 리포좀 제제 중 렉틴-엘라지탄닌 포합체의 분석법 확립
- ㆍ 저자명
- 전현주,최영욱,Jeon. Hyun-Joo,Choi. Young-Wook
- ㆍ 간행물명
- 藥劑學會誌
- ㆍ 권/호정보
- 2001년|31권 3호|pp.197-200 (4 pages)
- ㆍ 발행정보
- 한국약제학회
- ㆍ 파일정보
- 정기간행물| PDF텍스트
- ㆍ 주제분야
- 기타
Lectin-conjugated ellagitannin (LET), a newly introduced melanoma-specific antitumor agent which has been synthesized by conjugation of wheat germ agglutinin as a lectin with praecoxin A as an ellagitannin, was encapsulated into sterically stabilized liposomes (SSL). Modified Folin phenol method was established for the quantitation of LET contents in liposomal formulations protein employing the standard calibration curve with bovine serum albumin. After removal of phospholipid by organic solvent extraction, which interferes the specific selectivity of the Folin-Ciocalteu reagent with the protein, recovery of LET was $94.5{pm}2.3%$ and the encapsulation efficiency was revealed as $37.8{pm}5.9%$ for 2.5 mg/ml LET solution.