건강을 80% MeOH수용액으로 추출하고, 얻어진 추출물을 EtOAc, n-BuOH및 $H_2O$로 용매 분획하였다. 그 중에서 뚜렷한 활성을 보인 EtOAc 분획으로부터 DPPH(1-diphenyl-2-picryl hydrazyl) radical 포획법으로 항산화 활성을 추적하여 항산화 활성을 갖는 화합물 2종을 분리하였다. 각각을 NMR 및 MS 데이터의 해석과 문헌 자료를 조사하여 6-shogaol 및 12-shogaol로 구조를 결정하였다. 각 화합물의 $EC_{50}$값은 26.4 ${mu}M$과 22.5 ${mu}M$로 상용항산화제인 BHA, BHT 및 ${alpha}-tocopherol$보다 뚜렷이 높은 활성을 나타내었다.
The root of Zingiber officinale R. was extracted with 80% aqueous MeOH, and the concentrated extract was fractionated with EtOAc, n-BuOH and water phases. The EtOAc fraction exhibited most potent antioxidant activity among them, and two active compounds were isolated by activity-guided separation using repetitive silica gel column chromatography. On the bases of spectral data and the chemical characteristics, the structure of the compounds were determined as 6-shogaol and 12-shogaol, respectively. The $EC_{50}$ values of them were evaluated as 26.4 ${mu}M$ and 22.5 ${mu}M$, respectively, where these values were higher than the commercial antioxidants, such as, BHA, BHT and ${alpha}-tocopherol$.
