Objective: The present study has been undertaken to investigate the effects of Korean Carthami Semen and Estradiol-Carthami Semen co-treatment on osteoporosis in ovariectomized rats. Method: In this experiment, the rats were ovariectomized. Rats were administered 3 kinds of medicine, Korean Carthami Semen, Estradiol, and Estradiol-Carthami Semen, which were taken orally. The levels of bone mineral density, osteocalcin, estradiol, ALP, phosphorus, calcium in serum, deoxypyridinoline in urine, calcium, phosphorus, ash weight of bone, body weight and uterus weight were measured. Results: These experimetal results, it appears that the only Carthami Semen have more less efficacy on osteoporosis but $E_2$-Carthami Semen co-treated group showed more effective on osteoporosis. Conclusions: Further west-east medicine combination study should be conducted to illustrate in depth the curing osteoporosis.