Liner is usually used to prevent see-through of outer cloths and to improve slipperiness of the cloths and appearance of garment. Although liner should have basic characteristic such as air permeability, antistatic property, and dimensional stability during wear or cleaning, the demand for antimicrobial propertyis increasing recently for sanitary clothing environment. We polymer which is prepared in or laboratory. Regenerated cellulose and its blends were treated with the antimicrobial polymer via typical pad-curing method. Acetates were partially saponified by sodium hydroxide to generate cellulose hydroxyl group and then treated with the polymer. The antimicrobial properties of the liners and their fastness to laundering were evaluated by shake flask test.