A new bacteriocin produced by Bacillus licheniformis cy2 was partially purified and characterized. The bacteriocin named as BSCY2 was stable in the pH range of 2.5~9.5. BSCY2 was stable below 4$0^{circ}C$ and it retained its antimicrobial activity during long tern storage at -2$0^{circ}C$ and -7$0^{circ}C$. BSCY2 was inactivated 15 min exposure to temperatures over 8$0^{circ}C$ and lost 50% of its antimicrobial activity within 2 hr at 7$0^{circ}C$. BSCY2 was inactivated by proteinase K treatment, which indicates its proteinous nature. Direct detection of the BSCY2 band showing antimicobial activity on Tricine-SDS-PAGE suggested an apparent molecular mass of about 6,500 dalton. These characterizatics of BSCY2 are considered as potential compounds for use in bioindustry.